|
KMID : 0545120130230081107
|
|
Journal of Microbiology and Biotechnology
2013 Volume.23 No. 8 p.1107 ~ p.1115
|
|
|
Intermolecular Interaction Between Cry2Aa and Cyt1Aa and Its Effect on Larvicidal Activity Against Culex quinquefasciatus
|
|
Dennis K. Bideshi
Greer Waldrop
Maria Teresa Fernandez-Luna
Mercedes Diaz-Mendoza
Margaret C. Wirth
Jeffrey J. Johnson
Park Hyun-Woo
Brian A. Federici
|
|
|
Abstract
|
|
|
|
The Cyt1Aa protein of Bacillus thuringiensis susbp. israelensis elaborates demonstrable toxicity to mosquito larvae, but more importantly, it enhances the larvicidal activity of this species Cry proteins (Cry11Aa, Cry4Aa, and Cry4Ba) and delays the phenotypic expression of resistance to these that has evolved in Culex quinquefasciatus. It is also known that Cyt1Aa, which is highly lipophilic, synergizes Cry11Aa by functioning as a surrogate membrane-bound receptor for the latter protein. Little is known, however, about whether Cyt1Aa can interact similarly with other Cry proteins not primarily mosquitocidal; for example, Cry2Aa, which is active against lepidopteran larvae, but essentially inactive or has very low toxicity to mosquito larvae. Here we demonstrate by ligand binding and enzyme-linked immunosorbent assays that Cyt1Aa and Cry2Aa form intermolecular complexes in vitro, and in addition show that Cyt1Aa facilitates binding of Cry2Aa throughout the midgut of C. quinquefasciatus larvae. As Cry2Aa and Cry11Aa share structural similarity in domain II, the interaction between Cyt1Aa and Cry2Aa could be a result of a similar mechanism previously proposed for Cry11Aa and Cyt1Aa. Finally, despite the observed interaction between Cry2Aa and Cyt1Aa, only a 2-fold enhancement in toxicity resulted against C. quinquefasciatus. Regardless, our results suggest that Cry2Aa could be a useful component of mosquitocidal endotoxin complements being developed for recombinant strains of B. thuringiensis subsp. israelensis and B. sphaericus aimed at improving the efficacy of commercial products and avoiding resistance.
|
|
|
KEYWORD
|
|
|
Cry2Aa, Cyt1Aa, Bacillus thuringiensis subsp. israelensis, synergy, mosquito larvicide
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|
|